Differential pulse polarography: a method for the direct study of biosorption of metal ions by live bacteria from mixed metal solutions.

作者: Ioannis Savvaidis , Martin N. Hughes , Robert K. Poole

DOI: 10.1023/A:1025489915704

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摘要: The technique of differential pulse polarography is shown here to be applicable the monitoring directly biosorption metal ions from solution by live bacteria mixed solutions. Biosorption Cd(II), Zn(II) and Ni(II) P. cepacia was followed using data obtained at potential which characteristic ion in absence presence cells. Hepes buffer (pH 7.4, 50 mM) used as a supporting electrolyte polarographic chamber peaks cells lower amplitude were due metal-binding Well defined experiments involving mixtures Cd(II)-Zn(II), Cu(II)-Zn(II), Cu(II)-Cd(II) Cd(II)-Ni(II). increased with pH. method also tested rapid for assessing removal ability measuring solutions demonstrated. Cu(II) preferentially bound metals order > Zn(II), Cd(II) intact cepacia.

参考文章(23)
Dorothy L. Parker, Jennifer E. Mihalick, John L. Plude, Michelle J. Plude, Thomas P. Clark, Lynn Egan, Joshua J. Flom, L.C. Rai, H.D. Kumar, Sorption of metals by extracellular polymers from the cyanobacterium Microcystis aeruginosa fo. flos-aquae strain C3-40 Journal of Applied Phycology. ,vol. 12, pp. 219- 224 ,(2000) , 10.1023/A:1008195312218
Elizabeth A. Webster, Andrew J. Murphy, John A. Chudek, Geoffrey M. Gadd, Metabolism-independent binding of toxic metals by Ulva lactuca: cadmium binds to oxygen-containing groups, as determined by NMR Biometals. ,vol. 10, pp. 105- 117 ,(1997) , 10.1023/A:1018379106700
Ronald J. Doyle, Terrance J. Beveridge, Metal Ions and Bacteria ,(1989)
Robert K. Poole, Geoffrey M. Gadd, Metal-microbe interactions Published for the Society for General Microbiology by IRL Press. ,(1989)
T.J. Beveridge, M.N. Hughes, H. Lee, K.T. Leung, R.K. Poole, I. Savvaidis, S. Silver, J.T. Trevors, Metal-Microbe Interactions: Contemporary Approaches Advances in Microbial Physiology. ,vol. 38, pp. 177- 243 ,(1996) , 10.1016/S0065-2911(08)60158-7
R A Laddaga, S Silver, Cadmium uptake in Escherichia coli K-12 Journal of Bacteriology. ,vol. 162, pp. 1100- 1105 ,(1985) , 10.1128/JB.162.3.1100-1105.1985
Robert K. Poole, Martin N. Hughes, Metals and micro-organisms Chapman and Hall. ,(1989)
Louise Rome, Geoffrey M. Gadd, Use of pelleted and immobilized yeast and fungal biomass for heavy metal and radionuclide recovery Journal of Industrial Microbiology & Biotechnology. ,vol. 7, pp. 97- 104 ,(1991) , 10.1007/BF01576071