Degradation of triphenylmethane dyes using a temperature and pH stable spore laccase from a novel strain of Bacillus vallismortis

摘要: Abstract The characterization of a spore laccase from Bacillus vallismortis fmb-103, isolated textile industry disposal sites, is described. activity was 6.5 U/g dry with ABTS as the substrate. enzyme quite stable at high temperature. It retained more than 90% its initial after 10 h 70 °C. demonstrated broad pH stability in both acidic and alkaline conditions. There almost no loss 3 over an extended period time, relative remained 82% 38% 7 9 10 days. NaN , SDS, l -cysterine, Dithiothreitol, EDTA NaCl inhibit activity. Triphenylmethane dyes, including malachite green, brilliant green aniline blue were efficiently degraded by 24 h combination mediator efficiencies 76.84%, 96.56% 81.17%, respectively. reusability for decolorization dyes also examined.