Determination of the estrous cycle phases of rats: some helpful considerations

摘要: The short length of the estrous cycle rats makes them ideal for investigation changes occurring during reproductive cycle. lasts four days and is characterized as: proestrus, estrus, metestrus diestrus, which may be determined according to cell types observed in vaginal smear. Since collection secretion use stained material generally takes some time, aim present work was provide researchers with helpful considerations about determination rat phases a fast practical way. Vaginal thirty female collected every morning month unstained native using microscope without aid condenser lens. Using 10 x objective lens, it easier analyze proportion among three cellular types, are 40 recognize each one these types. lavage from animals, observation material, microscope, phase all took 15-20 minutes.