Separation of monosaccharides hydrolyzed from glycoproteins without the need for derivatization.

摘要: Chromatographic separation of monosaccharides hydrolyzed from glycoconjugates or complex, aggregate biomaterials, can be achieved by classic analytical methods without a need for derivatizing the monosaccharide subunits. A simple and sensitive method is presented characterizing underivatized following hydrolysis N- O-linked glycoproteins using high-performance liquid chromatography with mass spectrometry detection (LC-MS). This adaptable anything purified to mixtures glycoforms, relative absolute quantification applications, even analysis complex biomaterials. Use an amide stationary phase HILIC demonstrated retain highly polar, resolve stereoisomers potentially interfering contaminants. work illustrates original approach characterization glycoprotein standards, mixtures, biological materials such as total yeast extract.