Regulation of Female Folliculogenesis by Tsp1a in Nile Tilapia (Oreochromis niloticus).
作者： Mimi Jie , He Ma , Li Zhou , Jiahong Wu , Minghui Li
DOI: 10.3390/IJMS21165893
关键词:
摘要: TSP1 was reported to be involved in multiple biological processes including the activation of TGF-β signaling pathways and regulation angiogenesis during wound repair tumor growth, while its role ovarian folliculogenesis remains elucidated. In present study, Tsp1a found expressed oogonia granulosa cells phase I IV follicles ovaries Nile tilapia by immunofluorescence. tsp1a homozygous mutants were generated CRISPR/Cas9. Mutation resulted increased oogonia, reduced secondary growth delayed ovary development. Expression cell proliferation marker PCNA significantly up-regulated mutant ovaries. Furthermore, transcriptomic analysis revealed that expressions DNA replication related genes up-regulated, cAMP MAPK pathway which inhibit promote differentiation down-regulated. addition, aromatase (Cyp19a1a) expression serum 17β-estradiol (E2) concentration decreased mutants. These results indicated lacking inhibited turn, E2. Taken together, our essential for tilapia.
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sci-hub.st 参考文章(41)
Moh H. Malek, I. Mark Olfert, Global deletion of thrombospondin-1 increases cardiac and skeletal muscle capillarity and exercise capacity in mice. Experimental Physiology. ,vol. 94, pp. 749- 760 ,(2009) , 10.1113/EXPPHYSIOL.2008.045989
James Greenaway, Patricia A. Gentry, Jean-Jacques Feige, Jonathan LaMarre, Jim J. Petrik, Thrombospondin and Vascular Endothelial Growth Factor Are Cyclically Expressed in an Inverse Pattern During Bovine Ovarian Follicle Development Biology of Reproduction. ,vol. 72, pp. 1071- 1078 ,(2005) , 10.1095/BIOLREPROD.104.031120
James Greenaway, Jack Lawler, Roger Moorehead, Paul Bornstein, Jonathan LaMarre, Jim Petrik, Thrombospondin-1 inhibits VEGF levels in the ovary directly by binding and internalization via the low density lipoprotein receptor-related protein-1 (LRP-1) Journal of Cellular Physiology. ,vol. 210, pp. 807- 818 ,(2007) , 10.1002/JCP.20904
Nikolaos G. Frangogiannis, Guofeng Ren, Oliver Dewald, Pawel Zymek, Sandra Haudek, Anna Koerting, Kim Winkelmann, Lloyd H. Michael, Jack Lawler, Mark L. Entman, Critical Role of Endogenous Thrombospondin-1 in Preventing Expansion of Healing Myocardial Infarcts Circulation. ,vol. 111, pp. 2935- 2942 ,(2005) , 10.1161/CIRCULATIONAHA.104.510354
K.M.J. Menon, Bindu Menon, Structure, function and regulation of gonadotropin receptors – A perspective Molecular and Cellular Endocrinology. ,vol. 356, pp. 88- 97 ,(2012) , 10.1016/J.MCE.2012.01.021
H Kliem, H Welter, W D Kraetzl, M Steffl, H H D Meyer, D Schams, B Berisha, Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum. Reproduction. ,vol. 134, pp. 535- 547 ,(2007) , 10.1530/REP-06-0172
Yun-Lv Sun, Dong-Neng Jiang, Sheng Zeng, Chong-Jiang Hu, Kai Ye, Chao Yang, Shi-Jie Yang, Ming-Hui Li, De-Shou Wang, Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Nile tilapia (Oreochromis niloticus) Aquaculture. ,vol. 433, pp. 19- 27 ,(2014) , 10.1016/J.AQUACULTURE.2014.05.035
M. Luisa Iruela-Arispe, DeAnn J. Liska, E. Helene Sage, Paul Bornstein, Differential expression of thrombospondin 1, 2, and 3 during murine development Developmental Dynamics. ,vol. 197, pp. 40- 56 ,(1993) , 10.1002/AJA.1001970105
T. Kobayashi, H. Kajiura-Kobayashi, Y. Nagahama, Induction of XY sex reversal by estrogen involves altered gene expression in a teleost, tilapia. Cytogenetic and Genome Research. ,vol. 101, pp. 289- 294 ,(2003) , 10.1159/000074351
Azin Agah, Themis R. Kyriakides, Jack Lawler, Paul Bornstein, The lack of thrombospondin-1 (TSP1) dictates the course of wound healing in double-TSP1/TSP2-null mice. American Journal of Pathology. ,vol. 161, pp. 831- 839 ,(2002) , 10.1016/S0002-9440(10)64243-5