The interaction of sonochemically synthesized gold nanoparticles with serum albumins

作者: Selvaraj Naveenraj , Sambandam Anandan , Arunkumar Kathiravan , Rajalingam Renganathan , Muthupandian Ashokkumar

DOI: 10.1016/J.JPBA.2010.03.039

关键词:

摘要: Spherical gold nanoparticles of approximately 16nm were synthesized using a sonochemical reduction method and characterized UV-vis spectroscopy, atomic force microscopy (AFM) transmission electron (TEM). The binding these with bovine serum albumin (BSA) human (HSA) was investigated absorption fluorescence spectroscopic techniques. A strong quenching the from albumins observed due to formation ground state complex (static quenching). constants, number sites constants determined Stern-Volmer Benesi-Hildebrand plots. Using Forster Resonance Energy Transfer (FRET) theory, distance between donor (serum albumins) acceptor (gold nanoparticles) obtained, which showed that HSA has more affinity towards sonochemically compared other methods.

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