Killing of listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice.

摘要: Acquired resistance to the facultative intracellular bacterium Listeria monocytogenes is thought require immunologically activated macrophages. Using peritoneal exudate cells from nonimmunized mice in a suspension bactericidal assay, however, we found that neutrophils obtained early during inflammatory process (4 hr after elicitation) and macrophages later inflammation (maximal listericidal activity at 48 were able kill vitro. The kinetics of expression by against both L E coli similar. Although intraperitoneal immunization or intravenous hyperimmunization markedly enhanced vivo, did not increase ability phagocytes However, response injection proteose-peptone dead Listeria, immunized mobilized more monocytes into inflamed peritoneum. These data suggest that, rather than systemic activation mononuclear phagocyte activity, increased mobilization sites infection may be prime importance listeriosis.