Methods for extracting 'omes from microbialites
作者： Elizabeth Selene Gómez-Acata , Carla M. Centeno , Luisa I. Falcón
DOI: 10.1016/J.MIMET.2019.02.014
关键词:
摘要: Microbialites are organo-sedimentary structures formed by complex microbial communities that interact with abiotic factors to form carbonate rich fabrics. Extraction of DNA or total RNA from microbialites can be difficult because the high mineral concentration and exopolymeric substances. The methods employed until now include substances such as cetyltrimethylammonium bromide, sodium dodecyl sulfate, xanthogenate, lysozyme proteinase K, well mechanical disruption. Additionally, several commercial kits have been used improve extraction. This minireview presents different applied for extraction discusses their advantages disadvantages. Moreover, all 'omes (DNA, RNA, Protein, Lipids, polar metabolites) using multiomic (MPlex), state art viruses microbialites, also discussed.
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