Serologic detection of antibodies to Brucella spp. using a commercial ELISA in cattle in Grenada, West Indies.
作者： S. Kumthekar , H. Hariharan , K. Tiwari , D. Thomas , R. Sharma
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摘要: Bovine brucellosis, caused mainly by Brucella abortus, a zoonotic bacterium, has been reported from many areas of the world, including Central and South America, Caribbean island state Trinidad Tobago. Although brucellosis eradicated domestic cattle in Canada it still exists one or two herds United States. Serological tests are important estimating prevalence exposure order to target eradication programmes. In this study, serum samples 150 were tested using commercial competitive enzyme linked immunosorbent assay (SVANOVIR®Brucella-Ab C-ELISA) which detects antibodies both B. abortus melitensis. All greater than 6 months old unvaccinated. Sampled 35 representing animals all parishes Grenada. Nine (6%) positive for and/or melitensis C-ELISA. Of herds, 7 (20%) had C- ELISA-positive animals. Three contained herds. Based on high sensitivity (98%) specificity (99.7%) C-ELISA, these results strongly indicate presence exposed
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