The in vitro ToxTracker and Aneugen Clastogen Evaluation extension assay as a tool in the assessment of relative genotoxic potential of e-liquids and their aerosols.

摘要: In vitro (geno)toxicity assessment of electronic vapour products (EVPs), relative to conventional cigarette, currently uses assays, including the micronucleus and Ames tests. Whilst informative on induction a finite endpoint risk posed by test articles, such assays could benefit from mechanistic supplementation. The ToxTracker Aneugen Clastogen Evaluation analysis can indicate activation reporters associated with (geno)toxicity, DNA damage, oxidative stress, p53-related stress response protein damage. Here, we tested for different effects selection neat e-liquids, EVP aerosols Kentucky reference 1R6F cigarette smoke samples in assay. assay was initially validated assess whether mixture e-liquid base components, propylene glycol (PG) vegetable glycerine (VG) had interfering within system. This achieved spiking three positive controls into system PG/VG or phosphate-buffered saline bubbled (bPBS) aerosol (nicotine flavour free). did not greatly affect responses induced compounds. Next, when compared samples, e-liquids bPBS (tobacco flavour; 1.6% freebase nicotine, nicotine salt 0% nicotine) exhibited reduced less complex responses. Tested up 10% concentration, induce any reporters. Neat 1%, reporters, thought be due their osmolarity vitro. E-liquid content induced. Additionally, alone only an at supraphysiological level. conclusion, is quick, screen genotoxic potential mechanisms variety (compositionally complex) derived cigarettes EVPs. has future application battery next-generation (smoking alternative) products,