MerP/MerT-mediated mechanism: A different approach to mercury resistance and bioaccumulation by marine bacteria
作者： Jinlong Zhang , Yiting Zeng , Bing Liu , Xu Deng
DOI: 10.1016/J.JHAZMAT.2020.122062
关键词:
摘要: Abstract Currently, mechanism underlying mercury resistance and bioaccumulation of marine bacteria remains little understood. A bacterium Pseudomonas pseudoalcaligenes S1 is resistant to 120 mg/L Hg2+ with capacity 133.33 mg/g. Accordingly, was investigated at molecular cellular level. Annotation transcriptome reveals 772 differentially expressed genes, including Hg2+-relevant genes merT, merP merA. Both merT gene have three complete copies in genome, while merA has only one. In order evaluate the function these recombinant strains were constructed express MerA (named as A), MerT/MerP (TP) MerT/MerP/MerA (TPA), respectively. The results show that strain TP, TPA, are improved minimum inhibition concentration (MIC) being 60 mg/L, 40 mg/L, 20 mg/L, respectively compared 2 mg/L host strain. Strain TP TPA exhibit enhanced capacity, does not differ from control. Their equilibrium capacities 110.48 mg/g, 94.49 mg/g, 83.76 mg/g 82.29 mg/g, Summarily, different most microorganisms by MerA-mediated mechanism, achieves capability via MerT/MerP-mediated strategy.
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