Time resolved effect model for Daphnia magna – Measurement and modeling of the toxicokinetic phase of diazinon

摘要: The goal of this project is the development a time-resolved effect model for water flea Daphnia magna which describes toxic effects insecticides as function toxicokinetic and toxicodynamic processes. novelty approach lies in identification quantification insecticide its metabolites formed during phase inside organism. organophosphorous diazinon serves compound, chosen In presentation, we will show results analysis magna. consists passive uptake/ elimination into/ out organism, oxidation to active metabolite diazoxon by cytochrome P450, hydrolysis 2-isopropyl-6-methyl-4-pyrimidinol further processes catalysed different enzymes. themselves can be eliminated hydrolysis, conjugation or diffusion. vivo experiments was exposed diazinon. After exposure times internal concentration parent compound well were measured with HPLC-MS/MS. rate constants steps obtained modeling time course concentrations multi-component first-order kinetics model. that dominating step diazoxon. Diazoxon could only detected very low between limit detection quantification. A possible explanation are fast compared