Mutational-kinetic Analysis Reveals the Roles of Arginines and Aromatics in the Core of the GABAA Receptor Ligand-binding Pocket

Sabine W. Baumann, Roland Baur, Erwin Sigel, Forced Subunit Assembly in α1β2γ2 GABAAReceptors INSIGHT INTO THE ABSOLUTE ARRANGEMENT Journal of Biological Chemistry. ,vol. 277, pp. 46020- 46025 ,(2002) , 10.1074/JBC.M207663200

S O Casalotti, F A Stephenson, E A Barnard, Separate subunits for agonist and benzodiazepine binding in the gamma-aminobutyric acidA receptor oligomer. Journal of Biological Chemistry. ,vol. 261, pp. 15013- 15016 ,(1986) , 10.1016/S0021-9258(18)66821-8

Karen Bollan, Dale King, Laura A. Robertson, Kenneth Brown, Pamela M. Taylor, Stephen J. Moss, Christopher N. Connolly, GABAA Receptor Composition Is Determined by Distinct Assembly Signals within α and β Subunits Journal of Biological Chemistry. ,vol. 278, pp. 4747- 4755 ,(2003) , 10.1074/JBC.M210229200

D Benke, J M Fritschy, A Trzeciak, W Bannwarth, H Mohler, Distribution, prevalence, and drug binding profile of gamma-aminobutyric acid type A receptor subtypes differing in the beta-subunit variant. Journal of Biological Chemistry. ,vol. 269, pp. 27100- 27107 ,(1994) , 10.1016/S0021-9258(18)47131-1

N G Bowery, T G Smart, GABA and glycine as neurotransmitters: a brief history British Journal of Pharmacology. ,vol. 147, ,(2006) , 10.1038/SJ.BJP.0706443

Arthur Karlin, Myles H. Akabas, [8] Substituted-cysteine accessibility method Methods in Enzymology. ,vol. 293, pp. 123- 145 ,(1998) , 10.1016/S0076-6879(98)93011-7

S.M. Sine, P. Quiram, F. Papanikolaou, H.J. Kreienkamp, P. Taylor, Conserved tyrosines in the alpha subunit of the nicotinic acetylcholine receptor stabilize quaternary ammonium groups of agonists and curariform antagonists. Journal of Biological Chemistry. ,vol. 269, pp. 8808- 8816 ,(1994) , 10.1016/S0021-9258(17)37041-2

N. Kleinberger-Doron, B.I. Kanner, Identification of tryptophan residues critical for the function and targeting of the gamma-aminobutyric acid transporter (subtype A). Journal of Biological Chemistry. ,vol. 269, pp. 3063- 3067 ,(1994) , 10.1016/S0021-9258(17)42047-3

S. John Mihic, Qing Ye, Marilee J. Wick, Vladimir V. Koltchine, Matthew D. Krasowski, Suzanne E. Finn, Maria Paola Mascia, C. Fernando Valenzuela, Kirsten K. Hanson, Eric P. Greenblatt, R. Adron Harris, Neil L. Harrison, Sites of alcohol and volatile anaesthetic action on GABA(A) and glycine receptors. Nature. ,vol. 389, pp. 385- 389 ,(1997) , 10.1038/38738

Verena Tretter, Noosha Ehya, Karoline Fuchs, Werner Sieghart, Stoichiometry and Assembly of a Recombinant GABAA Receptor Subtype The Journal of Neuroscience. ,vol. 17, pp. 2728- 2737 ,(1997) , 10.1523/JNEUROSCI.17-08-02728.1997