AMASS: Software for Automatically Validating the Quality of MS/MS Spectrum from SEQUEST Results
作者： Wei Sun , Fuxin Li , Jue Wang , Dexian Zheng , Youhe Gao
DOI: 10.1074/MCP.M400120-MCP200
关键词:
摘要: Time-consuming and experience-dependent manual validations of tandem mass spectra are usually applied to SEQUEST results. This inefficient method has become a significant bottleneck for MS/MS data processing. Here we introduce program AMASS ( advanced spectrum screener), which can filter the results by measuring match percentage high-abundant ions continuity matched fragment in b, y series. Compared with Xcorr DeltaCn filter, increase number positives reduce negatives 22 datasets generated from 18 known protein mixtures. It effectively removed most noisy spectra, false interpretations, about half poor fragmentation work synergistically Rscore filter. We believe use result more accurate identification peptide time energy validation.
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