alpha-Actinin and vinculin in normal and thrombasthenic platelets.

摘要: Recently, the contractile protein alpha-actinin was identified in normal human platelets by its antigenic cross-reaction with a monospecific antibody to purified muscle alpha-actinin. In this study, we extend that preliminary identification of platelet Amino acid analysis, one-dimensional peptide maps, and silver stain analysis on polyacrylamide gels demonstrate alpha- actinin shows greater degree similarity smooth than striated There is no evidence suggest glycoprotein. addition, find thrombasthenic platelets, which are deficient glycoproteins IIb IIIa (GPIIb GPIIIa) contain amounts alpha-actinin, confirming recent finding GPIIIa different proteins platelets. We both also vinculin, 130,000-dalton polypeptide found many cell types at sites end-on attachment microfilaments plasma membrane. Thus, defect GPIIb does not diminish content either or vinculin.