Signalling and behaviour of Globodera pallida in the rhizosphere of the trap crop Solanum sisymbriifolium

摘要: Potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are economically important pests of potato (Solanum tuberosum) crops in growing regions worldwide. Integrated management is under threat, with effective nematicides increasingly being withdrawn on environmental health grounds. Alternative strategies urgently needed trap cropping could be one them. The non-tuber-bearing Solanum sisymbriifolium regarded as an crop for PCN strong hatching ability immunity to infection has been used the UK Netherlands. However, its mode action unknown. In order shed light so that a novel control strategy identified, interactions between pallida S. were investigated using vitro bioassays. choice assays, J2s equally attracted roots those tuberosum. root diffusate (PRD), which routinely induce hatch, failed attract chemotaxis bioassays, indicating factors (HFs) soluble compounds present PRD not involved attraction roots. invaded large numbers but develop further. To facilitate continuous observation nematode development, method was devised use Pluronic F-127, requires no sterilisation, life cycle successfully observed tuberosum Quantitative real-time polymerase chain reaction analyses defence related genes infected revealed up-regulation chitinase gene (ChtC 2.1) at 3 days post inoculation Electrospray ionisation-mass spectrometry exudate extracts two species subsequent bioassay-guided fractionation showed HF differs from Previously, attention had solely paid sisymbriifolium, this study first time aerial part extract possesses significant ability.