Microassay of O2 Evolution from Single Plant Cells

摘要: The measurement of O2 evolution in a closed system is one the easiest means demonstrating, or following process photosynthesis leaf, suspension leaf cells isolated chloroplasts. Conventional and widely applied techniques are Warburg manometry Clark-type oxygen electrodes (e.g. Delieu Walker 1972). For assay particle suspensions (cells, chloroplasts) volumes typically 1 ml employed, corresponding to, e.g. 105 to 106 mesophyll cells. With an ordinary electrode, as used for discs, lower limit detection corresponds change about nmol (Delieu 1981). This equivalent amount evolved by 103 104 per minute.