Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis
作者： Qi Wang , Yaokun Chen , Haijun Lu , Haiji Wang , Hui Feng
DOI: 10.1002/IUB.2242
关键词:
摘要: BACKGROUND Quercetin, a widely distributed bioflavonoid, plays role in combating diverse human cancers including non-small cell lung cancer (NSCLC). However, the of quercetin reversing radioresistance NSCLC cells and its underlying mechanism are far from being elucidated. METHOD Radiation-resistant lines were established. Quantitative real-time PCR (qRT-PCR) was used to detect expression miR-16-5p WEE1 G2 checkpoint kinase (WEE1) mRNA radiation-resistant cells. After treated with different concentrations doses X-ray, proliferation apoptosis monitored by CCK-8 assay, colony formation flow cytometry, respectively. Ultimately, targeting relationship between verified via dual fluorescent reporter gene assay. RESULTS The down-regulated cells, while up-regulated. Quercetin enhanced radiosensitivity dose- time-dependent manner. Furthermore, treatment increased decreased WEE1. function reversed inhibitors or transfection overexpressing plasmids. CONCLUSION In conclusion, modulating
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