Reassessing the Potential Activities of Plant CGI-58 Protein

摘要: Comparative Gene Identification-58 (CGI-58) is a widespread protein found in animals and plants. This has been shown to participate lipolysis mice humans by activating Adipose triglyceride lipase (ATGL), the initial enzyme responsible for triacylglycerol (TAG) catabolism cascade. Human mutation of CGI-58 cause Chanarin-Dorfman syndrome, an orphan disease characterized systemic accumulation TAG which engenders tissue disorders. The also neutral lipid metabolism plants and, this case, again provokes accumulation. Although its roles as ATGL coactivator are quite clear, catalytic activity still question. acyltransferase activities have speculated about, reported or even dismissed experimental evidence that expressed E. coli possesses unambiguous lacking. To address problem, we developed new set plasmids site-directed mutants elucidate vivo effects expression on coli. By analyzing composition selected strains expressing proteins, reinvestigating enzymatic tests with adequate controls, show here recombinant plant none proposed previously described. Recombinant mouse both lack towards either lysophosphatidylglycerol lysophosphatidic acid form phosphatidylglycerol phosphatidic does not catalyze phospholipid hydrolysis. However, CGI-58, but led decrease all tested, putative residues restored wild-type phenotype. potential subsequently discussed.