Effects of high glucose on the production of heparan sulfate proteoglycan by mesangial and epithelial cells

摘要: Changes in heparan sulfate metabolism may be important the pathogenesis of diabetic nephropathy. Recent studies performed on renal biopsies from patients with nephropathy revealed a decrease glycosaminoglycan staining glomerular basement membrane without changes for proteoglycan-core protein. To understand this phenomenon at cellular level, we investigated effect high glucose conditions synthesis proteoglycan by cells vitro. Human adult mesangial and visceral epithelial were cultured under normal (5 mM) (25 conditions. Immunofluorescence 25 mM confirmed extended vivo histological observations. Using metabolic labeling observed an altered production conditions, predominantly compared to dermatan or chondroitin proteoglycan. N-sulfation analysis produced less di- tetrasaccharides larger oligosaccharides, indicating sulfation pattern. Furthermore, quantification ELISA, significant was when We conclude that concentration induces alteration cells. absolute quantities are both explain earlier These importance integrity charge-dependent filtration barrier growth-factor matrix interactions