Fragmentation of the Golgi apparatus of motor neurons in amyotrophic lateral sclerosis revealed by organelle-specific antibodies.

摘要: Abstract Many studies have established the central involvement of Golgi apparatus in transport and processing plasma membrane, lysosomal, secreted proteins. The neurons is also involved axoplasmic flow fast-moving macromolecules orthograde, retrograde, transsynaptic exogenous ligands. Markers apparatus, based on traditional methods enzyme cytochemistry, are not applicable to human tissues obtained at autopsy. For that reason, brain cells has been examined adequately diseases nervous system. Here we report an antiserum raised against MG-160, a 160-kDa sialoglycoprotein medial cisternae several rat cells, specific easily reproducible immunocytochemical marker other Application this probe amyotrophic lateral sclerosis shown fragmentation motor similar induced by depolymerization microtubules. We suggest functional implications because significant reductions secretion insulin immunoglobulins observed islet respectively, treated with microtubule-disrupting agents.