Borrelia persica: In vitro cultivation and characterization via conventional PCR and multilocus sequence analysis of two strains isolated from a cat and ticks from Israel.
作者： Sandra Schwarzer , Gabriele Margos , Evelyn Overzier , Volker Fingerle , Gad Baneth
DOI: 10.1016/J.TTBDIS.2015.06.012
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摘要: Abstract Borrelia persica , one of the pathogenic agents tick-borne relapsing fever, is transmitted by soft tick Ornithodoros tholozani . It causes infections in humans as well animals. In this study, we developed a medium, termed Pettenkofer/LMU Bp, for reliable vitro cultivation. Cell densities up to 5.2 × 10 7 viable cells/ml were achieved over at least 40 passages. The cultivable B. strain isolated from cat was further analyzed amplification flaB gene using conventional PCR. addition, seven housekeeping genes ( clpA clpX, pepX pyrG recG rplB and uvrA) second out pooled ticks Israel amplified phylogenetic relationships among species analyzed. results PCR multilocus sequence analysis confirmed our isolates
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