Integrating metabolomics and targeted gene expression to uncover potential biomarkers of fungal/oomycetes-associated disease susceptibility in grapevine.

摘要: Vitis vinifera, one of the most cultivated fruit crops, is susceptible to several diseases particularly caused by fungus and oomycete pathogens. In contrast, other species (American, Asian) display different degrees tolerance/resistance these pathogens, being widely used in breeding programs introgress resistance traits elite V. vinifera cultivars. Secondary metabolites are important players plant defence responses. Therefore, characterization metabolic profiles associated with disease susceptibility grapevine a promising approach identify trait-related biomarkers. this work, leaf composition eleven genotypes was analysed using an untargeted metabolomics approach. A total 190 putative were found discriminate resistant/partial resistant from genotypes. The biological relevance discriminative compounds assessed pathway analysis. Several selected as biomarkers expression genes coding for enzymes their pathways analysed. Reference established normalisation candidate gene expression. leucoanthocyanidin reductase 2 (LAR2) presented significant increase genotypes, accordance catechin accumulation analysis group. Up our knowledge first time that constitutive proposed, opening new insights into selection on programs.