Production of monoclonal and polyclonal antibodies against human osteocalcin sequences and development of a two-site ELISA for intact human osteocalcin
作者： Ilpo Kuronen , Harri Kokko , Markku Parviainen
DOI: 10.1016/0022-1759(93)90127-S
关键词:
摘要: Abstract In this study we have raised polyclonal and monoclonal antibodies against human osteocalcin sequences coupled to bovine serum albumin (BSA). The were used for the development of a novel two-site ELISA on microtiter plates intact osteocalcin. epitope, recognized by hybridoma product Os 31/2 that was as capture antibody, located amino terminal sequence 1–29. detection 1–49. This method failed detect any tryptic peptides derived from 1–49 nor did it peptide 1–29, thus demonstrating high specificity only. is technique determination We also validated assay parameters routine use. direct relatively simple procedure promises be powerful diagnostic investigative tool.
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