Changes in the viral mRNA expression pattern correlate with a rapid rate of CD4+ T-cell number decline in human immunodeficiency virus type 1-infected individuals.

摘要: The rate of disease progression varies considerably among human immunodeficiency virus type 1 (HIV-1)-infected individuals. Several cross-sectional studies have shown an association between the stage HIV-1 and viral burden or relative levels gene expression. To study extent transcription replication its correlations with progression, we quantified serial, longitudinal samples blood cells from 10 HIV-1-infected individuals markedly different rates CD4+ T-cell number decline following seroconversion. After normalization for input nucleic acid content, multiply spliced mRNA unspliced RNA were by competitive reverse transcription-PCR using oligonucleotide primers which flank major tat/rev/nef splice junction span internal region gag open reading frame, respectively. Coamplification cRNA template controls was used to normalize variation in efficiency vitro enzymatic amplification. Similarly, proviral DNA also PCR performed within linear range Viral detected each individual all time points regardless decline. Unspliced genomic rapidly increased who had a precipitously declining number. In contrast, both mRNAs remained relatively stable benign clinical course. These data demonstrate that correlates quantifying intracellular is potential prognostic value.