A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells
作者： Bahar Baser , Johannes Spehr , Konrad Büssow , Joop van den Heuvel
DOI: 10.1016/J.YMETH.2015.11.022
关键词:
摘要: Stable mammalian production cell lines in suspension culture enable the reproducible expression of target genes any desired scale using bioreactor technology. Targeted integration methods have been developed to cut down timelines for generation stable producer lines. The powerful Flp recombinase mediated cassette exchange (RMCE) technique allows fast preselected and optimized high loci so called master Up now, these cells only from a single locus. Here, we describe set-up required multiple tagged on example binary RMCE system glycosylation mutant CHO Lec3.2.8.1 line. We show how this technology is used proteins by generating system. tools strategy construction with different combinations level are described detail. independent individual model protein tdTomato cumulative chosen combination This can be further multi co-expression complex subunits predetermined ratio each
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