Establishment of cell culture systems from penaeid shrimp and their susceptibility to white spot disease and yellow head viruses
作者： S. N. Chen , C. S. Wang
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摘要: Monolayer cultures were established from ovary, heart, lymphoid tissue and peripheral hemocytes of penaeid shrimps including Penaeus monodon, P. japonicus penicillatus. The most favorable conditions for the culture shrimp cells in vitro was CMRL L-15 media when used within an osmolarity range 620--760 mmol/kg. optimal maintenance temperature 25 °C tissues 28 monodon Among four tested, tissue, or 'Oka organ', superior to other formation confluent cell monolayers. Cell ovary have been subcultured up three times. When heart cultured, a maximum survival 4 days obtained. In contrast, derived maintained alive at least 20 appropriate systems. Neither sheet nor adherence obtained cultivation hepatopancreas using present results study also revealed that extract, muscle extract lobster hemolymph enhanced cultured vitro. organ' monolayer incubated with either white spot disease virus (WSDV) yellow head (YHV), no cytopathic effect (CPE) However, 5--7 after establishment, significant CPE (a few foci) observed monolayers WSDV- YHV-infected Oka tissue. By electron microscopy, virions WSDV YHV nuclei cytoplasm cells. foci developed further increased incubation time.
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D.V. Lightner, R.M. Redman, T.A. Bell, Infectious hypodermal and hematopoietic necrosis, a newly recognized virus disease of penaeid shrimp Journal of Invertebrate Pathology. ,vol. 42, pp. 62- 70 ,(1983) , 10.1016/0022-2011(83)90202-1
S. N. CHEN, G. H. KOU, Infection of cultured cells from the lymphoid organ of Penaeus monodon Fabricius by monodon‐type baculovirus (MBV) Journal of Fish Diseases. ,vol. 12, pp. 73- 76 ,(1989) , 10.1111/J.1365-2761.1989.TB01294.X
Lourdes M. Tapay, Yuanan Lu, Remedios B. Gose, Elpidio Cesar B. Nadala, J.A. Brock, P.C. Loh, Development of an in vitro quantal assay in primary cell cultures for a non-occluded baculo-like virus of penaeid shrimp. Journal of Virological Methods. ,vol. 64, pp. 37- 41 ,(1997) , 10.1016/S0166-0934(96)02142-8
J. Carl Barrett, Takeki Tsutsui, Paul O. P. Ts'o, Neoplastic transformation induced by a direct perturbation of DNA. Nature. ,vol. 274, pp. 229- 232 ,(1978) , 10.1038/274229A0
Donald V. Lightner, Rita M. Redman, A baculovirus-caused disease of the penaeid shrimp, Penaeus monodon Journal of Invertebrate Pathology. ,vol. 38, pp. 299- 302 ,(1981) , 10.1016/0022-2011(81)90137-3
Kiyoshi Inouye, Satoshi Miwa, Norihisa Oseko, Heiji Nakano, Takeshi Kimura, Kazuo Momoyama, Midori Hiraoka, Mass mortalities of cultured kuruma shrimp Penaeus japonicus in Japan in 1993: electron microscopic evidence of the causative virus. Fish Pathology. ,vol. 29, pp. 149- 158 ,(1994) , 10.3147/JSFP.29.149
C Chantanachookin, S Boonyaratpalin, J Kasornchandra, S Direkbusarakom, U Ekpanithanpong, K Supamataya, S Sriurairatana, TW Flegel, Histology and ultrastructure reveal a new granulosis-like virus in Penaeus monodon affected by yellow head disease Diseases of Aquatic Organisms. ,vol. 17, pp. 145- 157 ,(1993) , 10.3354/DAO017145
D.V. Lightner, R.M. Redman, T.A. Bell, Observations on the geographic distribution, pathogenesis and morphology of the baculovirus from Penaeus monodon Fabricius Aquaculture. ,vol. 32, pp. 209- 233 ,(1983) , 10.1016/0044-8486(83)90220-X
Heiji Nakano, Hiroshi Koube, Statoshi Umezawa, Kazuo Momoyama, Midori Hiraoka, Kiyoshi Inouye, Norihisa Oseko, Mass Mortalities of Cultured Kuruma Shrimp, Penaeus japonicus, in Japan in 1993 : Epizootiological Survey and Infection Trials Fish Pathology. ,vol. 29, pp. 135- 139 ,(1994) , 10.3147/JSFP.29.135
Kazuo Momoyama, Midori Hiraoka, Heiji Nakano, Hiroshi Koube, Kiyoshi Inouye, Norihisa Oseko, Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Histopathological study Fish Pathology. ,vol. 29, pp. 141- 148 ,(1994) , 10.3147/JSFP.29.141