Isolation of a new human fetal liver cytochrome P450 cDNA clone: Evidence for expression of a limited number of forms of cytochrome P450 in human fetal livers
作者： Masayuki Komori , Kanako Nishio , Tomomichi Fujitani , Hiroaki Ohi , Mitsukazu Kitada
DOI: 10.1016/0003-9861(89)90213-0
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摘要: Abstract From a human fetal liver cDNA library, new clone (λHFL10) was isolated using an antiserum to P450 HFLa, which has been from livers of fetuses. Cytochrome cDNAs, namely λhPA6, λhP2-1, and λhPD4 were highly homologous clones, pHY13, Hp1-1, phP450j, respectively, also the library adult livers. Using these clones as probes together with λHFL10, Northern blot analysis conducted determine whether all cytochromes expressed in The results clearly showed that only HFL10 mRNA detected This result supports allegation there is much more limited number forms cytochrome than
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