Effect of Ca2+ on the Amperometric Determination of Dehydrogenase Substrates with Nitro-Fluorenone Modified Electrodes
作者： Nicolas Mano , Patrice Peyrou , Alexander Kuhn
DOI: 10.1002/1521-4109(200105)13:8/9<770::AID-ELAN770>3.0.CO;2-J
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摘要: We report about the electrocatalytic properties of electrodes modified by adsorption nitro-fluorenone derivatives. use their high catalytic activity in electrooxidation NADH to enhance electron transfer kinetics between enzymes dehydrogenase family and electrode. The presence a carboxyl substituent mediator molecule allows us improve rate constants when adding calcium ions solution. This is due specific complexation NADH. interaction, as well molecular recognition NADH/NAD+ dehydrogenases used modify electrode surface “molecular lego” approach. obtained supramolecular sandwich (mediator/Ca2+/NAD+/dehydrogenase) able detect specifically various substrates function type dehydrogenase, constitutes therefore versatile approach for construction biosensors.
sci-hub.se 参考文章(14)
W.John Albery, Philip N. Bartlett, Derek H. Craston, Amperometric enzyme electrodes: Part II. Conducting salts as electrode materials for the oxidation of glucose oxidase Journal of Electroanalytical Chemistry. ,vol. 194, pp. 223- 235 ,(1985) , 10.1016/0022-0728(85)85006-3
Interaction of yeast alcohol dehydrogenase with various substrates. I. Binding of coenzymes. Journal of Biochemistry. ,vol. 74, pp. 971- 984 ,(1973) , 10.1093/OXFORDJOURNALS.JBCHEM.A130332
Ioanis Katakis, Elena Dom�nguez, Catalytic electrooxidation of NADH for dehydrogenase amperometric biosensors Mikrochimica Acta. ,vol. 126, pp. 11- 32 ,(1997) , 10.1007/BF01242656
N Mano, A Kuhn, Ca2+ enhanced electrocatalytic oxidation of NADH by immobilized nitro-fluorenones Electrochemistry Communications. ,vol. 1, pp. 497- 501 ,(1999) , 10.1016/S1388-2481(99)00097-1
Bernd Gründig, Gunther Wittstock, Ulrich Rüdel, Beate Strehlitz, Mediator-modified electrodes for electrocatalytic oxidation of NADH Journal of Electroanalytical Chemistry. ,vol. 395, pp. 143- 157 ,(1995) , 10.1016/0022-0728(95)04090-B
Paul. Pantano, Werner G. Kuhr, Dehydrogenase-modified carbon-fiber microelectrodes for the measurement of neurotransmitter dynamics. 2. Covalent modification utilizing avidin-biotin technology. Analytical Chemistry. ,vol. 65, pp. 623- 630 ,(1993) , 10.1021/AC00053A023
N Mano, A Kuhn, Cation induced amplification of the electrocatalytic oxidation of NADH by immobilized nitro-fluorenone derivatives Journal of Electroanalytical Chemistry. ,vol. 498, pp. 58- 66 ,(2001) , 10.1016/S0022-0728(00)00249-7
P. N. Bartlett, P. R. Birkin, E. N. K. Wallace, Oxidation of β-nicotinamide adenine dinucleotide (NADH) at poly(aniline)-coated electrodes Journal of the Chemical Society, Faraday Transactions. ,vol. 93, pp. 1951- 1960 ,(1997) , 10.1039/A700468K
N. Mano, A. Kuhn, Immobilized nitro-fluorenone derivatives as electrocatalysts for NADH oxidation Journal of Electroanalytical Chemistry. ,vol. 477, pp. 79- 88 ,(1999) , 10.1016/S0022-0728(99)00393-9
L. Gorton, Carbon paste electrodes modified with enzymes, tissues, and cells Electroanalysis. ,vol. 7, pp. 23- 45 ,(1995) , 10.1002/ELAN.1140070104