Prophage lambda at unusual chromosomal locations: I. Location of the secondary attachment sites and the properties of the lysogens

摘要: Abstract The integration, frequency of phage λ into a mutant host deleted for the normal prophage insertion site is reduced about 200-fold relative to integration wild-type Escherichia coli. This residual like requires int gene and occurs by cross-over at attachment on chromosome. Analysis resulting abnormal lysogens indicates that there are certain sites bacterial chromosome which preferentially utilized insertion. In addition, in has inserted or near specific gene, thereby inactivating its function, may be obtained appropriate selection technique. When been inactivated excision can restore function. Prophage from inefficient but, site, it two genes: xis. this respect resemble rather than hybrid site. value deletion mapping various portions E. coli production novel types transducing phages.