Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction.

摘要: Objective To explore the composition of ocular microbiome in normal subjects and patients with Meibomian gland dysfunction (MGD). Subjects methods Seventy (140 eyes) were enrolled our study. Signs dry eye evaluated bacterial species conjunctival sac (CS) (MG) secretions then identified by 16S rRNA gene sequencing. Additionally, 17 (34 further to determine differences microbiomes surface deep layers MG using a segmental secretion analysis. Results The positive isolation rate was markedly higher than CS. control mild group simple, whereas bacteria more complex as severity MGD increased. number types significantly severe those control, moderate groups. Corynebacterium macginleyi only detected group, an up 26.3%. Furthermore, new grading system for proposed appeared be positively correlated grade severity. analysis showed had incidence discordance rate. Conclusion rate, greater species, severity, which implied that might changes. This study provided some basis indications antibiotic clinical practice.