A Comparison of Three Circular Mitochondrial Genomes of Fagus sylvatica from Germany and Poland Reveals Low Variation and Complete Identity of the Gene Space

摘要: Similar to chloroplast loci, mitochondrial markers are frequently used for genotyping, phylogenetic studies, and population genetics, as they easily amplified due their multiple copies per cell. In a recent study, it was revealed that the offers little variation this purpose in central European populations of beech. Thus, aim study elucidate, if sequences might offer an alternative, or whether similarly conserved Europe. For purpose, circular genome sequence from more than 300-year-old beech reference individual Bhaga German National Park Kellerwald-Edersee assembled using long short reads compared Jamy Nature Reserve Poland recently published eastern Germany. The 504,730 bp, while genomes other two individuals were 15 bases shorter, seven indel locations, with four having three locations one base less Bhaga. addition, 19 SNP found, none which inside genes. these 17 different Bhaga, genomes, 2 had same Polish individual. While figures slightly higher genome, comparison confirms low degree genetic divergence organelle DNA Europe, suggesting colonisation common gene pool after Weichsel Glaciation. have limited use studies but once glacial refugia become available, be suitable pinpoint origin migration re-colonising population.