Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells
作者： Mahdi Mirahmadi , Saeideh Nakhaei-Rad , Maryam M. Matin , Mina Shahriyari , Morvarid Saeinasab
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摘要: Abstract Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, fusion, extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs valuable sources for drug screening human disease modeling. Alternatives to virus-based include application DNA-free methods chemically defined culturing conditions. However, the possibility tumor development is still a hurdle. By taking advantage NTERA-2 cells, embryonal carcinoma line, we obtained partially differentiated examined dedifferentiation capacity regenerative tissue rabbit ears. Results indicated that treatment with regenerating tissue-conditioned medium (CM) expression key pluripotency markers as real-time polymerase chain reaction, flow cytometry, immunocyt...
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sci-hub.se 参考文章(59)
P W Andrews, N C Dracopoli, J Føgh, D Simon, C Carlin, I Damjanov, G S Banting, Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Laboratory Investigation. ,vol. 50, pp. 147- 162 ,(1984)
Hitoshi Niwa, Jun-ichi Miyazaki, Austin G. Smith, Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. ,vol. 24, pp. 372- 376 ,(2000) , 10.1038/74199
Julin Xie, Shaohai Qi, Yingbin Xu, Jingming Tang, Tianzeng Li, Xusheng Liu, Bin Shu, HuiZhen Liang, Bing Huang, Effects of basic fibroblast growth factors on hypertrophic scarring in a rabbit ear model. Journal of Cutaneous Medicine and Surgery. ,vol. 12, pp. 155- 162 ,(2008) , 10.2310/7750.2008.07041
Apostolos Zaravinos, Giannoula Soufla, John Bizakis, Demetrios Spandidos, Expression analysis of VEGFA, FGF2, TGFβ1, EGF and IGF1 in human nasal polyposis Oncology Reports. ,vol. 19, pp. 385- 391 ,(2008) , 10.3892/OR.19.2.385
Richard J. Goss, L. Nichols Grimes, Epidermal downgrowths in regenerating rabbit ear holes Journal of Morphology. ,vol. 146, pp. 533- 542 ,(1975) , 10.1002/JMOR.1051460408
M. M. Matin, Specific Knockdown of Oct4 and 2-microglobulin Expression by RNA Interference in Human Embryonic Stem Cells and Embryonic Carcinoma Cells Stem Cells. ,vol. 22, pp. 659- 668 ,(2004) , 10.1634/STEMCELLS.22-5-659
Trond Aasen, Angel Raya, Maria J Barrero, Elena Garreta, Antonella Consiglio, Federico Gonzalez, Rita Vassena, Josipa Bilić, Vladimir Pekarik, Gustavo Tiscornia, Michael Edel, Stéphanie Boué, Juan Carlos Izpisúa Belmonte, Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nature Biotechnology. ,vol. 26, pp. 1276- 1284 ,(2008) , 10.1038/NBT.1503
Bruce M. Carlson, Some principles of regeneration in mammalian systems. The Anatomical Record Part B: The New Anatomist. ,vol. 287, pp. 4- 13 ,(2005) , 10.1002/AR.B.20079
Peter W. Andrews, Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro Developmental Biology. ,vol. 103, pp. 285- 293 ,(1984) , 10.1016/0012-1606(84)90316-6
Dohoon Kim, Chun-Hyung Kim, Jung-Il Moon, Young-Gie Chung, Mi-Yoon Chang, Baek-Soo Han, Sanghyeok Ko, Eungi Yang, Kwang Yul Cha, Robert Lanza, Kwang-Soo Kim, Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell. ,vol. 4, pp. 472- 476 ,(2009) , 10.1016/J.STEM.2009.05.005