Live Cell DNA Labeling and Multiphoton/Confocal Microscopy
作者： P SMITH , R ERRINGTON
DOI: 10.1016/B978-012164730-8/50038-1
关键词:
摘要: Publisher Summary Techniques for the discrimination and location of DNA, chromatin architecture, chromosomes, nuclear superstructures, cell nuclei in their various forms through cycle are increasing interest biosciences generation automated screening systems. Advanced microscopy methods such as multiphoton excitation laser scanning (MPLSM) now becoming more generally accessible, they provide significant advantages live research. The technique a fluorophore, using pulsed light source, provides solutions to several problems associated with continuous wave via single photon absorption. Multiphoton at wavelengths between 720 980 nm uses laser-scanning microscope comprising 1024 MP unit controlled by LaserSharp software (Bio-Rad Cell Science Division) attached Zeiss Axiovert 135. When considering labeling, performance status is most critical issue achieving optimal staining. There subtle shift violet red bias emission spectrum Hoechst 33342 upon DNA binding.
sci-hub.st 参考文章(16)
Winfried Denk, David W. Piston, Watt W. Webb, Two-photon molecular excitation in a laser scanning microscopy Springer US. pp. 445- 458 ,(1990) , 10.1007/978-1-4757-5348-6_28
P D Zalewski, I J Forbes, W H Betts, Correlation of apoptosis with change in intracellular labile Zn(II) using zinquin [(2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)acetic acid], a new specific fluorescent probe for Zn(II) Biochemical Journal. ,vol. 296, pp. 403- 408 ,(1993) , 10.1042/BJ2960403
Paul J. Smith, Nicola Blunt, Marie Wiltshire, Terence Hoy, Paul Teesdale-Spittle, Michael R. Craven, James V. Watson, W. Brad Amos, Rachel J. Errington, Laurence H. Patterson, Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry. ,vol. 40, pp. 280- 291 ,(2000) , 10.1002/1097-0320(20000801)40:4<280::AID-CYTO4>3.0.CO;2-7
Duncan H. Bell, Characterization of the fluorescence of the antitumor agent, mitoxantrone. Biochimica et Biophysica Acta. ,vol. 949, pp. 132- 137 ,(1988) , 10.1016/0167-4781(88)90063-2
Marius A. Albota, Chris Xu, Watt W. Webb, Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm Applied Optics. ,vol. 37, pp. 7352- 7356 ,(1998) , 10.1364/AO.37.007352
Tom Frey, Stephen Yue, Richard P. Haugland, Dyes providing increased sensitivity in flow-cytometric dye-efflux assays for multidrug resistance. Cytometry. ,vol. 20, pp. 218- 227 ,(1995) , 10.1002/CYTO.990200305
Tom Frey, Nucleic acid dyes for detection of apoptosis in live cells Cytometry. ,vol. 21, pp. 265- 274 ,(1995) , 10.1002/CYTO.990210307
Paul J. Smith, Margaret Lacy, Paul G. Debenham, James V. Watson, A mammalian cell mutant with enhanced capacity to dissociate a bis-benzimidazole dye-DNA complex. Carcinogenesis. ,vol. 9, pp. 485- 490 ,(1988) , 10.1093/CARCIN/9.3.485
Marc A.M.J. van Zandvoort, Cees J. de Grauw, Hans C. Gerritsen, Jos L.V. Broers, Mirjam G.A. oude Egbrink, Frans C.S. Ramaekers, Dick W. Slaaf, Discrimination of DNA and RNA in cells by a vital fluorescent probe: Lifetime imaging of SYTO13 in healthy and apoptotic cells Cytometry. ,vol. 47, pp. 226- 235 ,(2002) , 10.1002/CYTO.10076
Joseph R. Lakowicz, Ignacy Gryczynski, Henryk Malak, Martin Schrader, Peter Engelhardt, Hiroski Kano, Stefan W. Hell, Time-Resolved Fluorescence Spectroscopy and Imaging of DNA Labeled with DAPI and Hoechst 33342 Using Three-Photon Excitation Biophysical Journal. ,vol. 72, pp. 567- 578 ,(1997) , 10.1016/S0006-3495(97)78696-X