Heterologous Expression of Pleurotus eryngiiPeroxidase Confirms Its Ability To Oxidize Mn2+ and Different Aromatic Substrates

作者: Francisco Javier Ruiz-Dueñas , María Jesús Martínez , Ángel T. Martínez

DOI: 10.1128/AEM.65.10.4705-4707.1999

关键词:

摘要: A versatile ligninolytic peroxidase has been cloned from Pleurotus eryngii and its allelic variant MnPL2 expressed in Aspergillus nidulans, with properties similar to those of the mature enzyme P. eryngii. These include ability oxidize Mn2+ aromatic substrates, confirming that this is a new type sharing catalytic lignin manganese peroxidase.

参考文章(28)
E A Pease, A Andrawis, M Tien, Manganese-dependent peroxidase from Phanerochaete chrysosporium. Primary structure deduced from cDNA sequence. Journal of Biological Chemistry. ,vol. 264, pp. 13531- 13535 ,(1989) , 10.1016/S0021-9258(18)80029-1
T.L. Poulos, S.L. Edwards, H. Wariishi, M.H. Gold, Crystallographic refinement of lignin peroxidase at 2 A. Journal of Biological Chemistry. ,vol. 268, pp. 4429- 4440 ,(1993) , 10.1016/S0021-9258(18)53627-9
M Sundaramoorthy, K Kishi, M H Gold, T L Poulos, The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution. Journal of Biological Chemistry. ,vol. 269, pp. 32759- 32767 ,(1994) , 10.1016/S0021-9258(20)30056-9
P Stewart, R E Whitwam, P J Kersten, D Cullen, M Tien, Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Applied and Environmental Microbiology. ,vol. 62, pp. 860- 864 ,(1996) , 10.1128/AEM.62.3.860-864.1996
A T Smith, N Santama, S Dacey, M Edwards, R C Bray, R N Thorneley, J F Burke, Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme. Journal of Biological Chemistry. ,vol. 265, pp. 13335- 13343 ,(1990) , 10.1016/S0021-9258(19)38303-6
D Pribnow, M B Mayfield, V J Nipper, J A Brown, M H Gold, Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Journal of Biological Chemistry. ,vol. 264, pp. 5036- 5040 ,(1989) , 10.1016/S0021-9258(18)83695-X
Robin A. Lockington, H.M. Scaly-Lewis, C. Scazzocchio, R.Wayne Davies, Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans Gene. ,vol. 33, pp. 137- 149 ,(1985) , 10.1016/0378-1119(85)90088-5
Sovan Sarkar, Angel T Martı́nez, Marı́a Jesús Martı́nez, Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus Biochimica et Biophysica Acta. ,vol. 1339, pp. 23- 30 ,(1997) , 10.1016/S0167-4838(96)00201-4