Functional chicken gizzard heavy meromyosin expression in and purification from baculovirus-infected insect cells

摘要: Abstract The heavy chain and the essential regulatory light chains of chicken gizzard meromyosin (HMM) were coexpressed in Spodoptera frugiperda (fall armyworm) cells infected with a mixture two recombinant Autographa californica baculoviruses. Soluble HMM consisting types was obtained. The purified from virus-infected characterized. isolated phosphorylated by myosin kinase presence calmodulin Ca(2+)-dependent manner. ATPase activated rabbit skeletal muscle actin when kinase, calmodulin, Ca2+ present reaction medium. Chicken tropomyosin enhanced actin-activated activity. decorated filaments, displaying characteristic arrowhead pattern along filaments. This report on functional double-headed smooth fragment opens way to detailed studies molecule.