Genetic variability in regenerated plants of Ungernia victoris

作者: O. M. Bublyk , I. O. Andreev , K. V. Spiridonova , V. A. Kunakh

DOI: 10.1007/S10535-012-0106-2

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摘要: To determine the suitability of micropropagation techniques developed for conserving rare medicinal herb Ungernia victoris we estimated genetic fidelity plants produced through direct regeneration from bulb scale segments and organogenesis long-term callus culture. Average value Jaccard's distances between explant-derived regenerants maternal calculated RAPD data was 0.5 %, while that callus-derived cell line 4.2 %; average objects among were 0.7 % 2.5 respectively. The obtained suggest conditions in vitro culture applied this work provide relatively high stability species upon cultured callus.

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