Polyacrylamide Gel Electrophoresis of Viral Proteins

摘要: Publisher Summary This chapter describes polyacrylamide gel electrophoresis of viral proteins. Viral structural and nonstructural proteins are agents expression for the information carried in nucleic acid genomes. An understanding behavior a virus requires its be characterized. For this purpose characterization acids, gels is most sensitive versatile method. The genetic content number viruses relatively small compared even to simple cells. Two aspects as supporting media zonal experiments responsible dramatic separations. First, anti-convection gels, they more homogeneous stable than other commonly used materials. Second, with correct choice concentrations, spaces matrix similar dimensions macromolecules so that selective sieving occurs during electrophoretic migration. Gels composed extensively at present time.