Molecular gut analysis of carabids (Coleoptera: carabidae) using aphid primers
作者： Sarah Kate. Wallace
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摘要: Carabid beetles are found in a variety of Montana crops, although their impact on the food web is poorly known. To detect aphidophagy using polymerase chain reaction (PCR), carabids were fed single aphid and allowed to digest for increasing time intervals. Aphidspecific PCR primers used amplify DNA from carabid beetles. For laboratory-fed beetles, detection decreased with longer digestion periods. Further, there differences among genera proportion positive at tested Field surveys 273 individual beetle gut contents, representing seven genera, all surveyed cropping systems, except Microlestes Pasymachus, DNA. Genera Calosoma, Harpalus, Pterostichus, Amara, Agonum, Bembidion. The detectability prey context predator size discussed.
montana.edu参考文章(82)
M. E. G. Evans, The carabid body plan: a functional interpretation Springer, Dordrecht. pp. 25- 31 ,(1994) , 10.1007/978-94-017-0968-2_4
Robert T. Allen, The Occurrence and Importance of Ground Beetles in Agricultural and Surrounding Habitats Springer, Dordrecht. pp. 485- 505 ,(1979) , 10.1007/978-94-009-9628-1_27
S. M. Hill, J. M. Crampton, DNA-based methods for the identification of insect vectors. Annals of Tropical Medicine and Parasitology. ,vol. 88, pp. 227- 250 ,(1994) , 10.1080/00034983.1994.11812864
Hans-Ulrich Thiele, Relationships between Annual and Daily Rhythms, Climatic Demands and Habitat Selection in Carabid Beetles Springer, Dordrecht. pp. 449- 470 ,(1979) , 10.1007/978-94-009-9628-1_24
Fabián D. Menalled, Jana C. Lee, Douglas A. Landis, Manipulating carabid beetle abundance alters prey removal rates in corn fields Biocontrol. ,vol. 43, pp. 441- 456 ,(1999) , 10.1023/A:1009946004251
Paul D. Allison, Logistic Regression Using the SAS System : Theory and Application ,(1999)
W. den Boer-Daanje, P.J. den Boer, On life history tactics in carabid beetles: are there only spring- and autumn-breeders? pp. 247- 258 ,(1990)
Thierry Hance, Relationships between crop types, carabid phenology and aphid predation in agroecosystems 7th European Carabidologists' Meeting held at the Royal Entomological Society in London. ,(1990)
Thierry Hance, Predation impact of carabids at different population densities on Aphis fabae development in sugar beet Pedobiologia. ,vol. 30, pp. 251- 262 ,(1987)
Marlijn Hoogendoorn, George E. Heimpel, PCR-based gut content analysis of insect predators: using ribosomal ITS-1 fragments from prey to estimate predation frequency. Molecular Ecology. ,vol. 10, pp. 2059- 2067 ,(2001) , 10.1046/J.1365-294X.2001.01316.X