Further results in understanding the subpopulation structure of AML: clonogenic cells and their progeny identified by differentiation markers.

摘要: We performed a subpopulation analysis in acute myelocytic leukemia (AML) with the objective of relating phenotypic diversity to clonogenic properties cells. AML-CFUs considered as representatives precursor cell compartment were phenotyped for surface markers by use eight monoclonal antibodies directed against myeloid differentiation antigens and Ia antigen. Thus, specifically depleted (in complement-dependent lysis) or selected fluorescence-activated sorting), cells inoculated into colony culture. report here that AML-CFU phenotypes showed considerable variability among seven patients AML studied. The heterogeneous regard antigen density on represented distinct population leukemias. immunophenotypes did not differ consequence variations techniques, ie, different stimulatory materials. marrow blood, shown four subjects, contained identical maturation stages. In addition, direct cellular offspring phenotyped. This was done analyzing colonies produced culture, it apparent their antigenic compatible later stages differentiation. For example, all granulocytic B4.3-negative, whereas progeny significant numbers B4.3-positive evidence indicating progenitors descendant are identified features corroborates concept structure human is reflection capabilities precursors. fact variably expressed even lacking has clinical importance immunodiagnosis residual immunoseparation from autologous bone grafts.