Polymerase Chain Reaction-Based K-ras Mutation Detection of Pancreatic Adenocarcinoma in Routine Cytology Smears

摘要: The cytologic diagnosis of pancreatic carcinoma is notoriously difficult, particularly in distinguishing benign atypia from well-differentiated adenocarcinoma. Mutation codon 12 the K-ras oncogene frequently found with cancers. Detection by polymerase chain reaction (PCR) followed restriction endonuclease digestion can provide a powerful tool to improve and confirm diagnosis. authors examined utility PCR-based detection using routinely obtained cytology smears that could be collected at most hospitals. Pancreatic were retrospectively 60 patients. DNA was extracted slides amplified PCR mismatched primers generated Bst-N1 recognition site wild type but not mutant allele. Results compared clinical follow-up. mutations observed 44 46 (95.7%) cases cancer, nor 2 islet cell tumor. amplification steps proved robust sensitive, capable detecting alleles contained only small foci suspicious cells. Our results indicate mutation analysis done reliably within 1 days routine without special handling, increasing sensitivity ambiguous while maintaining cost-effective relatively noninvasive sampling strategy.