摘要: The design and characterization of a fully automated portable capillary waveguide biosensor are discussed in this chapter. Highly specific target recognition is achieved through hybridization fluid-borne single-stranded DNA sequences extracted from natural targets to the complimentary nucleic acid sequence (“capture probe”) bound inner surface capillary. product enumerated use fluorescent labeling. A novel instantaneous normalization scheme based on two photodetectors, together with standard reference material, enables independent measurements by instrument. probability false-positive detection quantified development error rate. instrument exhibits low limits (~10−13 M) repeatability 6%. sensor can be rearmed denaturing step allowing for sequential over an extended time period.
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