Bioprocess Design for the Microbial Production of Natural Phenolic Compounds by Debaryomyces hansenii

摘要: Debaryomyces hansenii NRRL Y-7426 metabolised ferulic acid into different phenolic compounds using a factorial design where glucose concentration (in the range of 1–20 g/L), peptone (2–20 g/L) and yeast extract (0.2–10 g/L) were independent variables. The interrelationship between dependent operational variables was well fitted (R 2 > 0.95) to models including linear, interaction quadratic terms. Depending on nitrogen concentrations, which redirected metabolism, major degradation products 1,226.2 mg 4-vinyl guaiacol/L after 72 h (molar yield 86.0 %), 1,077.8 mg vanillic acid/L 360 h 91.1 %) or 1,682.6 mg acetovanillone/L 408 h 98.8 %) in fermentations carried out with 2,000 mg acid/L. Other metabolites such as vanillin, vanillyl alcohol 4-ethylguaiacol present lower amounts.