Fluorescent Chemosensors for Divalent Zinc Based on Zinc Finger Domains. Enhanced Oxidative Stability, Metal Binding Affinity, and Structural and Functional Characterization
作者： Grant K. Walkup , Barbara Imperiali
DOI: 10.1021/JA9642121
关键词:
摘要: The design, synthesis, and characterization of a family peptides modeled after the zinc finger domains, which has led to production fluorescent peptidyl sensor for divalent with enhanced oxidative stability, are reported. chemosensor design comprises synthetic template covalently attached reporter is sensitive metal-induced conformational changes in polypeptide construct. modular approach employed construction these chemosensors allows independent modification metal coordination sphere group. structural, fluorescence, binding properties effects integrating various environment fluorophores, 4-(dimethylamino)benzamide, 5-(dimethylamino)naphthalenesulfonamide, 3-carboxamidocoumarin, described. Manipulation ligand sphere, by removal one pair thiolate ligands, was undertaken enhance stability ...
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sci-hub.se 参考文章(40)
Fluorescent chemosensors for ion and molecule recognition American Chemical Society. ,(1993) , 10.1021/BK-1993-0538
Lisa M. Utschig, Jeffrey G. Wright, Thomas V. O'Halloran, Biochemical and spectroscopic probes of mercury(II) coordination environments in proteins. Methods in Enzymology. ,vol. 226, pp. 71- 97 ,(1993) , 10.1016/0076-6879(93)26006-U
Aaron Klug, John W. R. Schwabe, Protein motifs 5. Zinc fingers. The FASEB Journal. ,vol. 9, pp. 597- 604 ,(1995) , 10.1096/FASEBJ.9.8.7768350
S. F. Michael, V. J. Kilfoil, M. H. Schmidt, B. T. Amann, J. M. Berg, Metal binding and folding properties of a minimalist Cys2His2 zinc finger peptide. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 4796- 4800 ,(1992) , 10.1073/PNAS.89.11.4796
W. McIntire, D. Wemmer, A. Chistoserdov, M. Lidstrom, A new cofactor in a prokaryotic enzyme: tryptophan tryptophylquinone as the redox prosthetic group in methylamine dehydrogenase Science. ,vol. 252, pp. 817- 824 ,(1991) , 10.1126/SCIENCE.2028257
R.B. Thompson, M.W. Patchan, Lifetime-based fluorescence energy transfer biosensing of zinc. Analytical Biochemistry. ,vol. 227, pp. 123- 128 ,(1995) , 10.1006/ABIO.1995.1260
John R. Jefferson, John B. Hunt, Ann Ginsburg, Characterization of indo-1 and quin-2 as spectroscopic probes for Zn2(+)-protein interactions. Analytical Biochemistry. ,vol. 187, pp. 328- 336 ,(1990) , 10.1016/0003-2697(90)90465-L
Kankan. Bhattacharyya, Mihir. Chowdhury, Environmental and magnetic field effects on exciplex and twisted charge transfer emission Chemical Reviews. ,vol. 93, pp. 507- 535 ,(1993) , 10.1021/CR00017A022
Erhard Hohenester, Christoph Kratky, Bernhard Kraeutler, Low-temperature crystal structure of superoxocobalamin obtained by solid-state oxygenation of the B12 derivative cob(II)alamin Journal of the American Chemical Society. ,vol. 113, pp. 4523- 4530 ,(1991) , 10.1021/JA00012A022
Marko. Virta, Jorma. Lampinen, Matti. Karp, A Luminescence-Based Mercury Biosensor Analytical Chemistry. ,vol. 67, pp. 667- 669 ,(1995) , 10.1021/AC00099A027