Biocontrol Efficacy Among Strains of Pochonia chlamydosporia Obtained from a Root-Knot Nematode Suppressive Soil.
作者： Jiue-in Yang , Angelo Loffredo , James Borneman , J. Ole Becker
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摘要: Three Pochonia chlamydosporia var. strains were isolated from a Meloidogyne incognita-suppressive soil, and then genetically characterized with multiple Pochonia-selective typing methods based on analysis of s-tubulin, rRNA internal transcribed spacer (ITS), small subunit (SSU), enterobacterial repetitive intergenic consensus (ERIC) PCR. All exhibited different patterns the ERIC analysis. Strains 1 4 similar PCR s-tubulin ITS. The strains' potential as biological control agents against root-knot nematodes examined in greenhouse trials. three P. significantly reduced numbers nematode egg masses. When chlamydospores used inoculum, strain tomato roots by almost 50%, showed effects J2 nematode-caused root-galling. A newly developed SSU-based differentiated others, could therefore potentially be screening tool for identifying other effective biocontrol chlamydosporia.
参考文章(35)
R.S. Hussey, K.R. Barker, A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique Plant disease reporter. ,vol. 57, pp. 1025- 1028 ,(1973)
Brian R. Kerry, Penny R. Hirsch, Ecology of Pochonia chlamydosporia in the rhizosphere at the population, whole organism and molecular scales Springer, Dordrecht. pp. 171- 182 ,(2011) , 10.1007/978-1-4020-9648-8_7
Martin E. Feder, Thomas Mitchell-Olds, Evolutionary and ecological functional genomics. Nature Reviews Genetics. ,vol. 4, pp. 649- 655 ,(2003) , 10.1038/NRG1128
Penny R. Hirsch, Simon D. Atkins, Tim H. Mauchline, C. Oliver Morton, Keith G. Davies, Brian R. Kerry, Methods for studying the nematophagous fungus Verticillium chlamydosporium in the root environment Plant and Soil. ,vol. 232, pp. 21- 30 ,(2001) , 10.1023/A:1010373717186
C. Gil-Lamaignere, E. Roilides, J. Hacker, F.-M.C. Müller, Molecular typing for fungi—a critical review of the possibilities and limitations of currently and future methods Clinical Microbiology and Infection. ,vol. 9, pp. 172- 185 ,(2003) , 10.1046/J.1469-0691.2003.00649.X
Marie Hallin, Alexander W. Friedrich, Marc J. Struelens, spa typing for epidemiological surveillance of Staphylococcus aureus Methods of Molecular Biology. ,vol. 551, pp. 189- 202 ,(2009) , 10.1007/978-1-60327-999-4_15
Annemie Hoedekie, Nicole Viaene, Veerle Van Damme, Long-term efficacy of Pochonia chlamydosporia for management of Meloidogyne javanica in glasshouse crops Nematology. ,vol. 7, pp. 727- 736 ,(2005) , 10.1163/156854105775142973
D.K. Arora, P.R. Hirsch, B.R. Kerry, PCR-based molecular discrimination of Verticillium chlamydosporium isolates Fungal Biology. ,vol. 100, pp. 801- 809 ,(1996) , 10.1016/S0953-7562(96)80025-6
CO Omwega, IJ Thomason, PA Roberts, A Nondestructive Technique for Screening Bean Germ Plasm for Resistance to Meloidogyne incognita Plant Disease. ,vol. 72, pp. 970- 972 ,(1988) , 10.1094/PD-72-0970
B.R. Kerry, F. Irving, J.C. Hornsey, Variation between strains of the nematophagous fungus, Verticillium chlamydosporium Goddard. I. Factors affecting growth in vitro. Nematologica. ,vol. 32, pp. 461- 473 ,(1986) , 10.1163/187529286X00336