Assessment of potential doubling time (Tpot), argyrophilic nucleolar organizer regions (AgNOR), and proliferating cell nuclear antigen (PCNA) as predictors of therapy response in canine non-Hodgkin's lymphoma

摘要: The predictive potential of several proliferation indices for therapeutic outcome was investigated in 55 dogs with spontaneously occurring non-Hodgkin's lymphoma (NHL). Indices included doubling time (Tpot), argyrophilic nucleolar organizer region (AgNOR) frequency, and proliferating cell nuclear antigen labeling index (PCNA-LI). All tumors were intermediate- or high-grade histology as assessed by the Working Formulation, all presented disease advanced clinical stage. treated an identical chemotherapeutic protocol. Tpot determination a bromodeoxyuridine (BrdU) delayed-biopsy technique readily applied dog. AgNOR frequency PCNA-LI easily obtained from archival, formalin-fixed, paraffin-embedded canine tissues. When accounting other prognostic variables employing multivariate analysis, (p=0.017), (p=0.021), but not PCNA-LI, first remission duration. (p=0.033) also survival time, approached significance (p=0.076). We conclude that can be used predictors NHL, spontaneous NHL dog may have significant model further characterization association between tumor kinetics chemoresponsiveness.