Analysis of Hardening of the Egg Envelope (Chorion) of the Fish, Oryzias latipes. (Egg envelope (chorion)/Egg activation/Chorion hardening/Fish egg/Chorion proteins)
作者： Kaori Masuda , Ichiro luchi , Kenjiro Yamagami
DOI: 10.1111/J.1440-169X.1991.00075.X
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摘要: Hardening of the chorion medaka eggs was quantitated in terms solubility its constituent proteins. After activation unfertilized with Ca2+-ionophore A23187, hardening (named ionophore-activation hardening) proceeded and 60 min after proteins 1 N NaOH had decreased to 20% that unhardened chorions. On SDS-PAGE, chorions gave four major protein bands (150, 83, 78 51 K). activation, new two (135 61 K) appeared, concurrent disappearance all original except K band. Isolated were also hardened by Ca2+and addition Ca2+the their about 45% originally. During this type ‘Ca2+-hardening), however, SDS-PAGE pattern remained unchanged. Therefore, there are mechanisms hardening. The ‘ionophore-activation inhibited cadaverine. When isolated 20 kept Ca2+-free conditions, process arrested: further resumed on Ca2+to medium. These results suggest presence some machinery
sci-hub.se 参考文章(33)
Tadashi S. Yamamoto, Some Morphological and Physiological Aspects of the Eggs of Teleostean Fishes 北海道大學理學部紀要 = JOURNAL OF THE FACULTY OF SCIENCE HOKKAIDO UNIVERSITY Series ⅤⅠ. ZOOLOGY. ,vol. 13, pp. 484- 488 ,(1957)
Norio Yoshizaki, Chiaki Katagiri, Necessity of Oviducal Pars Recta Secretions for the Formation of the Fertilization Layer in Xenopus laevis(Developmental Biology) Zoological Science. ,vol. 1, pp. 255- 264 ,(1984)
鷹司 岩松, Changes of the chorion upon fertilization in the medaka,Oryzias latipes 愛知教育大学研究報告 自然科学編. pp. 43- 56 ,(1969)
Mamoru Kusa, Significance of the Cortical Change in the Initiation of Development of the Salmon Egg (Physiological Analysis of Fertilization in the Egg of the Salmon,Oncorhynchus keta II) 日本動物学彙報. ,vol. 26, pp. 73- 77 ,(1953)
Toki-o Yamamoto, Physiology of fertilization in fish eggs. International Review of Cytology-a Survey of Cell Biology. ,vol. 12, pp. 361- 405 ,(1962) , 10.1016/S0074-7696(08)60545-8
Robin A. Wallace, Kelly Selman, Oogenesis in Fundulus heteroclitus. I. Preliminary observations on oocyte maturation in vivo and in vitro. Developmental Biology. ,vol. 62, pp. 354- 369 ,(1978) , 10.1016/0012-1606(78)90222-1
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A. I. Zotin, The mechanism of hardening of the salmonid egg membrane after fertilization or spontaneous activation. Development. ,vol. 6, pp. 546- 568 ,(1958) , 10.1242/DEV.6.4.546
R. E. Wyrick, T. Nishihara, J. L. Hedrick, Agglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibian Xenopus laevis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 71, pp. 2067- 2071 ,(1974) , 10.1073/PNAS.71.5.2067
C. A. Foerder, B. M. Shapiro, Release of ovoperoxidase from sea urchin eggs hardens the fertilization membrane with tyrosine crosslinks. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 4214- 4218 ,(1977) , 10.1073/PNAS.74.10.4214