Microfiltration of cutinase and Escherichia coli cell fragment suspensions: The role of the electrolyte environment on the development of dynamic cake layers

摘要: Abstract Stirred cell microfiltration of industrial relevant enzyme solutions, with and without debris, was studied. The biological system used a recombinant intracellular cutinase in suspensions Escherichia coli fragments. In NaCl forms large soluble protein aggregates that vary size the electrostatic microenvironment. Filtration shear forces applied on these break interaction among monomers, allowing 100% transmission to permeate. Pure solutions showed permeation flux reduction (10–34% simple electrolyte filtration runs) caused by adsorption aggregated molecules, leading membrane fouling formation dynamic cake layers. Cell homogenates presented severe decrease (range 93–97%), similar values, still dependent colloidal presence led debris concentration polarisation layers less resistant flow. experimental results were evaluated accordance aggregation behaviour cutinase, average zeta potential particles membrane.