Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors

摘要: Studies using animal models demonstrated the importance of autocrine/paracrine factors secreted by preimplantation embryos and reproductive tracts for embryonic development implantation. Although in vitro fertilization-embryo transfer (IVF-ET) is an established procedure, there no evidence that present culture conditions are optimal human early development. In this study, key polypeptide ligands known to be important were tested their ability improve embryo blastocyst outgrowth vitro. We confirmed expression ligand/receptor pairs cleavage derived from discarded tri-pronuclear zygotes endometrium. Combined treatment with growth (brain-derived neurotrophic factor, colony-stimulating epidermal granulocyte macrophage insulin-like factor-1, glial cell-line artemin) serum-free media promoted >2.5-fold blastocysts. For normally fertilized embryos, day 3 surplus cultured individually showed >3-fold increases 6–8 cell stage blastocysts >7-fold increase proportion high quality based on Gardner’s criteria. Growth factor also led a 2-fold promotion when 7 hatching used. When failed-to-be-fertilized oocytes used perform somatic nuclear (SCNT) fibroblasts as donor karyoplasts, inclusion increased progression reconstructed SCNT >4-cell embryos. supplementation cultures could promote implantation IVF-ET procedures. This approach valuable infertility future derivation patient-specific stem cells.