Competitive yeast action against Aspergillus carbonarius growth and ochratoxin A production.

摘要: Abstract The aim of the present study was to investigate interaction between 67 different yeast isolates and 3 wild Aspergillus carbonarius originated from Greek vineyards characterized by high ochratoxigenic potential. selected fungi were used either as single cultures or combined in a mixed culture. Yeasts grown mono-cultures co-cultures solid (MEA, CYA) liquid (CY broth) media, grape berries sterilized juice. Fungal growth monitored means colony area measurements. model Baranyi Roberts further fitted data provide estimates rate (cm2/day). Moreover, OTA analysis undertaken for CY broth agar well juice, on 8th 15th days incubation at 25 °C. A significant reduction fungal rate, final size toxin production observed both media species assayed. most competitive strains belonged Saccharomyces, Pichia, Metschnikowia, Dekkera Rhodotorula genera. Similar results obtained inoculated Specifically, Saccharomyces cerevisiae Y33 resulted decrease >90% 93% after 4 days co-culture, respectively. OTA, where concentration highest producer (A. F3) reduced 14,983 31,565 ng/mL 8 15 days, respectively, 5 ng/mL below detection limit (1 ng/g) when co-cultured with S. Y33. this could pool that must be investigated potential application biological control agents pre- post-harvest level wine juice processing.